ecl select western blotting detection reagent Search Results


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ecl plus western blotting detection system  (GE Healthcare)
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chemiscope 6200 gel imager  (Clinx Science)
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chemiluminescence ecl western blotting detection kit  (GE Healthcare)
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GE Healthcare chemiluminescence ecl western blotting detection kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Chemiluminescence Ecl Western Blotting Detection Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecl western blotting detection reagent  (GE Healthcare)
96
GE Healthcare ecl western blotting detection reagent
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Ecl Western Blotting Detection Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemiscope imager  (Clinx Science)
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Clinx Science chemiscope imager
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Chemiscope Imager, supplied by Clinx Science, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecl western blotting reagents  (GE Healthcare)
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GE Healthcare ecl western blotting reagents
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Ecl Western Blotting Reagents, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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amersham ecl prime western blotting detection reagent  (GE Healthcare)
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Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Amersham Ecl Prime Western Blotting Detection Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecl select western blotting detection reagent  (GE Healthcare)
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GE Healthcare ecl select western blotting detection reagent
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
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chemiscope 6200 touch chemiluminescence imaging system  (Clinx Science)
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Clinx Science chemiscope 6200 touch chemiluminescence imaging system
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
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chemiluminescence apparatus  (Clinx Science)
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Clinx Science chemiluminescence apparatus
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Chemiluminescence Apparatus, supplied by Clinx Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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amersham ecl start western blotting detection reagent  (GE Healthcare)
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GE Healthcare amersham ecl start western blotting detection reagent
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
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Image Search Results


Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with a monoclonal mouse antibody, clone 303.9 (ARP), and ECL (Amersham Pharmacia).

Journal:

Article Title: Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5- rep Sequence in cis

doi: 10.1128/JVI.76.11.5411-5421.2002

Figure Lengend Snippet: Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with a monoclonal mouse antibody, clone 303.9 (ARP), and ECL (Amersham Pharmacia).

Article Snippet: AAV Rep 78 protein was detected with a monoclonal mouse antibody supernatant, clone 303.9 (ARP), and an enhanced chemiluminescence (ECL) Western blotting detection kit (Amersham Pharmacia), according to the manufacturer's instructions.

Techniques: Expressing, Infection, Purification